Journal: International Journal of Biological Sciences

Article Title: Exosomal Transfer of Vasorin Expressed in Hepatocellular Carcinoma Cells Promotes Migration of Human Umbilical Vein Endothelial Cells

doi: 10.7150/ijbs.11943

Figure Lengend Snippet: VASN protein secretion and localization. (A) Western blot analysis of VASN protein in cell extracts and supernatant of HepG2 cells. (B) Electron micrograph of exosomes isolated from supernatants of HepG2 cells. Bar represents 100 nm. (C) VASN expression in exosomes, supernatant, and exosomes-depleted supernatants. The exosomal marker protein CD63 was also detected. (D) Western blot analysis of VASN protein in exosomes derived from HepG2, L02, and HUVECs. CD63 was used as a loading control. (E) Western blot analysis of VASN protein in cell lysates (top panel) and exosomes (bottom panel) from HepG2 cells transfected with NC or VASN siRNA (siVASN). GAPDH and CD63 were used as loading controls for cell lysates and exosomes, respectively.

Article Snippet: Rabbit polyclonal anti-CD63 antibody was purchased from Abcam, USA.

Techniques: Western Blot, Isolation, Expressing, Marker, Derivative Assay, Control, Transfection

Journal: Cancer Biology & Therapy

Article Title: Small extracellular vesicle-mediated ITGB6 siRNA delivery downregulates the αVβ6 integrin and inhibits adhesion and migration of recipient prostate cancer cells

doi: 10.1080/15384047.2022.2030622

Figure Lengend Snippet: Characterization of prostate cancer cell-derived small Extracellular Vesicles (sEVs) . (a) IB analysis (reducing conditions) for expression of β6 subunit, ALIX, TSG101, CD9, in PC3 total cell lysate, DU145 total cell lysate (TCL) and lysates from density gradient-isolated DU145 small extracellular vesicles (sEVs) in fractions one to ten. (b) Nanoparticle tracking analysis (NTA) of the PC3 and DU145 cell-derived, density gradient-isolated sEVs pooled from fractions one to five (sEV F1-F5). (c) IB analysis for expression of CD63, CD81, CANX (non-reducing conditions), and β6 subunit, ALIX, TSG101, CD9 (reducing conditions) in PC3 TCL, PC3 sEVs (F1-F5), DU145 TCL and DU145 sEVs (F1-F5).

Article Snippet: The following primary antibodies (Abs) were used for IB analyses: goat polyclonal Ab against the αVβ6 integrin (R&D Systems, AF2389); mouse monoclonal Abs against: αVβ6 integrin (Biogen, 6.2A1), ALIX (Abcam, ab117600), AR (Santa Cruz, sc-7305), CD9 (Santa Cruz, sc-13118), CD63 (Abcam, ab8219), CD81 (Abcam, ab23505); rabbit monoclonal Abs against β5 integrin subunit (Cell Signaling, 3629), TSG101 (Abcam, ab125011); rabbit polyclonal Abs against: ACTIN (Sigma Aldrich, A2066), Calnexin (CANX) (Cell Signaling, 2433S), and rabbit antiserum against αV subunit (C-terminus).

Techniques: Derivative Assay, Expressing, Isolation